Journal:

Article Title: Human Urine Contains Small, 150 to 250 Nucleotide-Sized, Soluble DNA Derived from the Circulation and May Be Useful in the Detection of Colorectal Cancer

doi:

Figure Lengend Snippet: Wild-type and mutant K-ras sequences detected in human urine and disease tissue by restriction-enriched polymerase chain reactions (RE-PCR). A: The predicted pattern of digested products. B: The results of analysis of DNA derived from the urine of an individual with no diagnosis of cancer (Zu) and from tumor tissue (AAt), non-tumor tissue that was 1 cm apart from the tumor (AAadj), serum (AAs), and urine (AAu) from an individual with CRC. C: The sensitivity and specificity of the assay by analysis of the reconstruction standards (1.5, 15, and 150 copies of SW480 genome per 50 ng of HepG2 DNA), and 50 ng HepG2 DNA and 5 ng SW480 alone, as indicated. D: Detection of mutated K-ras DNA in CRC tissue. CRC disease tissue sections from five CRC patients (Ot, AAt, AMt, BQt, and DGt) were subjected to DNA isolation and the RE-PCR assay for mutated K-ras DNA. MW: DNA MW markers. This represents the data from three independent experiments.

Article Snippet: One ml of resin (Wizard DNA Isolation Kit, Promega, Madison, WI) was added into the urine lysate and incubated for 2 hours at room temperature with gentle mixing.

Techniques: Mutagenesis, Derivative Assay, Biomarker Discovery, DNA Extraction